Human Tumor Liver Tumor Lysate

Name Human Tumor Liver Tumor Lysate
Supplier ABBIOTEC
Catalog 450220
Category Tissue Lysate
Prices $175.00
Sizes 100 µg
Applications WB
Species Human
Tissue Liver
Disease Tumor
Description Liver tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
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