Name | Human Tumor Liver Tumor Lysate |
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Supplier | ABBIOTEC |
Catalog | 450220 |
Category | Tissue Lysate |
Prices | $175.00 |
Sizes | 100 µg |
Applications | WB |
Species | Human |
Tissue | Liver |
Disease | Tumor |
Description | Liver tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. |
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